TABLE 1.

Oligonucleotide primers designed and used in this study

Primer	Size of product (bp)	Primer use(s)	Sequence (5′-3′)	Temp (°C)
narF	400	Isolation of narB fragment	ACNGGYCARCCYAAYGCNATG
narR		Isolation of narB fragment	CKHCKYTCNSWRTTNGTCAT
ntcAFor	462	Isolation of ntcA fragment, RT-PCR	GAACGCAACAAGACGATTTTCTT	57.10
ntcARev		Isolation of ntcA fragment, RT-PCR	GCTTCGGCAATGGCTTGATG	59.40
glnAFor	405	RT-PCR	CACCGGTGAACCTCGTCTAT	59.99
glnARev		RT-PCR	TCGATCCAGTTGTCGATGAA	60.20
narBFor	352	RT-PCR	GTTGGGTTACACCGAGCAGT	60.04
narBRev		RT-PCR	AGCACCAATGGGTAGGTGTC	59.85
nirAFor	288	RT-PCR	TCGAAAACTCTCCACGCTCT	60.13
nirARev		RT-PCR	GGGAAACGCTGCAATAATGT	59.97
nrtPFor	244	RT-PCR	CCTGACCTTTGTGGTCTGGT	60.00
nrtPRev		RT-PCR	GAACACCAGGATCGACGAGT	60.12
rbcLFor	193	RT-PCR	CACCGACCTCGACTTCTACA	58.88
rbcLRev		RT-PCR	TGCGAGTTGATGTTCTCGTC	59.99
nrtPtsp	NAa	Primer extension	GTGAGGTGAAGGGTTCGGTA	59.40
nirAext	NA	Primer extension	CCCGGGGACCTTTGTCGCTA	63.50
narBtsp	NA	Primer extension	TCACGGCTTGACCTTTCAC	56.70

^aNA, not applicable.