TABLE 1.
X. fastidiosa strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| X. fastidiosa | ||
| Temecula | Wild type, PD | ATCC 700964 |
| KLN59.3 | Gfp+, Kanr | This study |
| Plasmids | ||
| pGEM-5Zf(+) | Ampr | Promega |
| pKLN2 | X. fastidiosa oriC in pGEM-5Zf(+), Ampr | This study |
| pKLN41 | X. fastidiosa predicted untranscribed region in pGEM-5Zf(+), Ampr | This study |
| pKLN43 | X. fastidiosa predicted untranscribed region, X. fastidiosa oriC, Ampr | This study |
| pFAK | kan-2 source | 10 |
| pKLN56 | X. fastidiosa kan-2 integration allelic exchange plasmid, X. fastidiosa oriC, Ampr Kanr | This study |
| pPROBE-gfp[tagless] | gfp and transcriptional terminator source, Kanr | 22 |
| pKLN59 | X. fastidiosa kan-gfp integration allelic exchange plasmid, X. fastidiosa oriC, Ampr Kanr | This study |
a
Ampr and Kanr, resistance to ampicillin and kanamycin, respectively.