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Synthetic oligonucleotides used in this study
| Name | DNA sequence (5′ to 3′) |
|---|---|
| OriC F PvuI | CTCGATCGAGACTGGGATAAACTAATGCGAA |
| OriC R PvuI | ATCGATCGATGCGCTGTAATCTGAAACGCATTTGGT |
| Untxb2 F PstI | AACTGCAGACCGTCGTGAAAAGGGAAACAACC |
| Untxb2 R PstI | AACTGCAGACAAACACCGCCGTCGATATGAACTCTT |
| Kan-2 F RI Sma | GGAATTCCCGGGCTGTCTCTTATACACATCTCAACCA |
| Kan-2 R MCS | ATATCAATTGAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCAACCAATTCTGATTAGAAAAACTCA |
