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. 2011 Jan;133(1):88–95.

Table I.

List of primers used in the simplex PCR for the detection of virulence associated genes in B. cereus

Gene Primer (5’ →3’) Amplicon (bp)
hblA B component of haemolysin BL
HBLA1:GTGCAGATGTTGATGCCGAT 320
HBLA2:ATGCCACTGCGTGGACATAT
hblD L1 component of haemolysin BL
LIA:AATCAAGAGCTGTCACGAAT 430
L1B:CACCAATTGACCATGCTAAT
hblC L2 component of haemolysin BL
L2A:AATGGTCATCGGAACTCTAT 750
L2B:CTCGCTGTTCTGCTGTTAAT
nheA A component of non-haemolytic ET
nheA 344S: TACGCTAAGGAGGGGCA 500
nheA 843A: GTTTTTATTGCTTCATCGGCT
nheB B component of non-haemolytic ET
nheB 1500S:CTATCAGCACTTATGGCAG 770
nheB 2269A:ACTCCTAGCGGTGTTCC
nheC C component of non-haemolytic ET
nheC 2820S:CGGTAGTGATTTGCTGGG 582
nheC 3401A:CAGCATTCGTACTTGCCAA