Figure 6. Facilitation of neutrophil-mediated bacterial killing by sera from immunized mice.

Mice were treated as in figure 3, and heat-inactivated sera were incubated in vitro with P. aeruginosa, external complement, and neutrophils for two hours, then plated on tryptic soy agar for determination of cfu. n = 5 mice/group, with each serum sample assayed in duplicate; purified antibody control performed in triplicate. ^#,*significantly different than other groups, p<0.05.