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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2011 Mar 5;50(11):1536–1545. doi: 10.1016/j.freeradbiomed.2011.02.034

Fig 5. Immunological detection of the DNA-derived radical adduct from the reaction of calf thymus DNA with CuCl2 /H2O2 in the presence of DMPO.

Fig 5

Reaction mixtures were incubated at 37°C for 1 hr and then analyzed as described in experimental procedures with IST detection. No significant adduct formation was observed in the control experiments where one of the components was omitted. All components of the reaction must be present for DNA-adduct formation.