TABLE 2.
Summary of putative carotenoid biosynthesis genes found in the M. extorquens AM1 genome sequencea
| Gene(s) | Protein in GenBank with lowest E value | BLASTP E value | % Identity | Accession no. |
|---|---|---|---|---|
| crtB | R. capsulatus phytoene synthase | 1e-50 | 46 | P17056|CRTB_RHOCA |
| orf1 | R. capsulatus phytoene desaturase (dehydrogenase) | 1e-121 | 47 | P17054|CRTI_RHOCA |
| zdsA | Anabaena sp. zeta-carotene desaturase | 3e-66 | 32 | D26095 |
| crtZ | R. capsulatus phytoene desaturase | 2e-61 | 31 | P17054/CRTI_RHOCA |
| Gap of 4 putative ORFs | ||||
| crtI | Erwinia herbicola phytoene desaturase | 2e-50 | 31 | AAA64981 |
| crtB | Targetus erecta (marigold) phytoene synthase | 5e-22 | 37 | AAM45379 |
| orf2 | Streptomyces coelicolor putative phytoene dehydrogenase | 0.003 | 24 | NP_630834 |
| crtD (incomplete sequence) | Rubrivax gelatinosus methoxyneurosporene dehydrogenase | 3e-70 | 44 | AAC44798 |
| crtE | R. sphaeroides GGPP synthase | 7e-51 | 42 | P54976|CRTE_RHODSH |
| crtF | R. sphaeroides methoxyneurosporene methyltransferase | 4e-49 | 39 | T50751 |
| ispD-ispF | IspD-IspF (see Table 1) | |||
| ddsA | Paracoccus zeaxanthinifaciens decaprenyl diphosphate synthase (quinone biosynthesis) | 4e-84 | 54 | CAD24417 |
| ispA | R. sphaeroides FPP synthase | 6e-43 | 46 | BAA96457 |
| crtC (incomplete sequence) | R. sphaeroides hydroxyneurosporene synthase | 8e-12 | 42 | QO167055|CRTC_RHOSH |
a
Abbreviations are given in Fig. 1. Genes are grouped as they are clustered in the genome, with empty lines separating clusters. Enzymes used for the functional assignment are those found by a BLAST search of the translated gene sequence yielding the lowest E value.