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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Mol Cell Cardiol. 2010 Jan 4;49(1):70–78. doi: 10.1016/j.yjmcc.2009.12.013

Figure 4. Western blot of TNF-α in LV tissue and isolated cardiomyocytes of 24 hour ACF.

Figure 4

Pro- and active TNF-α in (A) heart lysates and (B) cardiomyocytes from ACF vs. shams. “BL” loading dye. “+” recombinant active rat TNF-α. Densitometric analysis is presented below. *P < 0.05, **P < 0.01 vs. sham. (C) Western blot of vimentin in cardiomyocytes and heart lysates (positive for vimentin) to ensure the purity of cardiomyocytes. Tubulin or calsequestrin was used as loading control.