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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Pediatr Res. 2011 Apr;69(4):271–278. doi: 10.1203/PDR.0b013e31820efbcf

Figure 9. Caffeine blocks hypoxia-induced HIF1 protein accumulation in hypoxic embryos.

Figure 9

Caffeine treatment inhibited HIF1 protein accumulation in hypoxic embryos. Dams were injected with normal saline or 20 mg/kg caffeine and then placed immediately in hypoxia (10% O2) or left in room air (21% O2). Embryos were collected after 6 hr, and whole embryo protein was isolated. Western blot analysis of HIF1 protein expression demonstrated a 40% reduction in stabilized HIF1 protein in caffeine-treated embryos exposed to hypoxia. β-Actin protein was examined on the same Western blots as a loading control. Reprinted from Wendler CC et al. FASEB J 23:1272– 1278; Copyright c 2009 FASEB, with permission.