Table 1.
AaOr expression survey in olfactory organs by RT–PCR. Positive expression is highlighted in grey. The number of (+) symbols represents the number of positive signals for each individual biological replicate performed; (–) negative results in any of the three biological replicates; nd, not determined. Additional information regarding these analyses may be found in the supporting text
| Gene | Adult antenna | Adult proboscis | Adult palps | Larval antenna | Forward primer | Reverse primer | |||
|---|---|---|---|---|---|---|---|---|---|
| male | female | male | female | male | female | ||||
| AaGPRor1 | - | - | - | - | - | - | +++ | ACGGGAGATCGAGAGAACAG | TGGTAGGAAGATCTGCGTC |
| AaGPRor2 | +++ | +++ | - | - | - | - | +++ | TGCTTGGCTCGATTTTGAC | AACCCTGCGGAGCAATG |
| AaGPRor3 | +++ | +++ | +++ | +++ | - | - | +++ | ACGCTGTTTGCTTGGAAC | TTCATAACGACGTAAGCCG |
| AaGPRoH | +++ | +++ | +++ | +++ | - | - | - | TCATTCTCTGCTACTACGGTG | ACGTCCGAATGGTTATGG |
| AaGPRor5 | +++ | +++ | +++ | +++ | - | - | - | TCATTCTCTGCTACTACGGTG | AAGTAGGACCATGATGTTTGC |
| AaGPRor6N | +++ | +++ | +++ | +++ | - | - | - | ACCAGTTCCGGACAATTATC | TGAAATACGACCACGATGC |
| AaGPRor7 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | AGAAAGCGATGACCATATCC | TTCAACTGCACCAACACC |
| AaGPRor8 | - | - | - | - | +++ | +++ | +++ | ACGGAAACAAATTGATTGAAGC | TGGCCAGCTCGAGTCATC |
| AaGPRor9 | - | - | - | - | - | - | +++ | TAGTGGACCATGGATTGAAC | TCATAGGATAGACGTTACCCAC |
| AaGPRor10 | +++ | +++ | - | - | - | - | - | TCCTGCTGATAATTCTACGGG | ATAAACCCGACGCAGC |
| AaGPRor11 | +++ | +++ | - | - | - | - | - | ACGACTGGTATAACTATCCCCG | AGCTTGTTTCAGGATCGTG |
| AaGPRor12C | nd | nd | nd | nd | nd | nd | nd | ||
| AaGPRor13 | +++ | +++ | - | - | - | - | - | TGTTTGAACGGAACTGCTCTC | TCGTTGAGCCCGTTGAATC |
| AaGPRor14 | - | - | - | - | - | - | ++ | TGGATTGCGTACTCATATGG | TGTGATACCTTCTTTGCGTTG |
| AaGPRor15 | +++ | +++ | +++ | +++ | - | - | +++ | TCGTGGCACTTACCGG | ACTTTGCTGCTGTGATACCTTC |
| AaGPRor16 | +++ | +++ | - | - | - | - | +++ | TGGATCTACCTGTTCAACGG | TATTCCTGCGCTGTTACAAG |
| AaGPRor17 | +++ | +++ | - | - | - | - | - | AGCCTCATCCCAAATTTTC | TCTCTTTAACGTGCTGGGAC |
| AaGPRor18C | nd | nd | nd | nd | nd | nd | nd | ||
| AaGPRor19 | +++ | +++ | - | - | - | - | - | AGGAACGCTCTTATTGATAAAGG | TCTCCATGTTCATAACTCCG |
| AaGPRor20 | +++ | +++ | - | - | - | - | - | TCCTTGCTACTTCTCAGTCTCCG | AGCAGCATAATTCCGGAGTAAAC |
| AaGPRor21 | +++ | +++ | - | - | - | - | - | TTTACTTCTGAACGGTGCCC | AGCATGCAAATCGAATACAGAG |
| AaGPRor22P | - | +++ | - | - | - | - | - | AGCAAAGGACATTGATGAGG | ACGCAGATGTGAGCTATGG |
| AaGPRor23 | +++ | +++ | - | - | - | - | - | ACTGGCACTCATTCTCAACTC | CTAGTTGAAACTCTGGAGCATTG |
| AaGPRor24 | +++ | +++ | - | - | - | - | - | AGGAGATTTGGAGGTGGATG | TCCCGTTGAAGTTGTTCG |
| AaGPRor25 | - | - | - | - | - | - | - | ACCGGATAAGATCTATGCTCTCG | TGGAGTAGTACGAAAATGCCG |
| AaGPRor26 | +++ | +++ | - | - | - | - | - | AGTTTTTCCACCTCAACCG | TGACCTTGGTCTCGATCTTC |
| AaGPRor27 | +++ | +++ | - | - | - | - | - | TCGTGCATATTCCAGACCTG | TCCACTGACCACTCGTACC |
| AaGPRor28 | - | - | - | - | - | - | - | TTCCAAGTGTTTGCTCAGG | TTGAGCCCGTTGTCCAC |
| AaGPRor29P | +++ | +++ | - | - | - | - | +++ | ACCGAATGCCCTCAACGTGATG | ATGGTTGTAGATGGCATCGCTAACC |
| AaGPRor30 | - | - | - | - | - | - | - | TTCGTCTACTCCATGCTCG | TAGGATGATTCGTTGTTCGC |
| AaGPRor31 | +++ | +++ | - | - | - | - | - | TGCGGGCTCAAGTTC | AGCAACGTACGAGCCAG |
| AaGPRor32P | - | - | - | - | - | - | - | TGGTCTACTCTATGTTGAGCACC | TTGCCAGTAGTAAGCTTCGC |
| AaGPRor33 | +++ | +++ | - | - | - | - | - | TGGTGCTATTATGGAAACAGAG | ACTTGAATGGCTTCTGCG |
| AaGPRor34 | - | - | - | - | - | - | +++ | AACGACCGGAAAGATCTG | ACCGAATAGGCTGTGTTTAGAG |
| AaGPRor35C | nd | nd | nd | nd | nd | nd | nd | ||
| AaGPRor36 | - | +++ | - | - | - | - | - | TCGGTTGTGCGTTATTCTAC | AGGTCGCACAATTGTATCG |
| AaGPRor37 | - | - | - | - | - | - | +++ | TCCAAGATATCGAACGAGTCTAC | ACAACAGGTACATCATCATCCC |
| AaGPRor38P | +++ | +++ | - | - | - | - | - | ACGGCTTGTTCATCGGAGTTTGTC | ATGCCCAGCGAGTACCACAC |
| AaGPRor39N | +++ | +++ | - | - | - | - | - | GGTGACGGTTTGTTCATCGGAGTTT | CGGTAGCGATACCAGGGAAAATCGT |
| AaGPRor40 | - | - | - | - | - | - | +++ | ACCGCTAAAGCTAACCGC | TTAACTAGTATGGCGACTACCG |
| AaGPRor41 | +++ | +++ | - | - | - | - | - | TTGAAAGCCAACAGCGAC | ATTAAGCGTCATCATTCCTCC |
| AaGPRor42 | - | - | - | - | - | - | - | AATGACGCTGTTTGTGCTAC | ATATCGTACACCTCCTGGATG |
| AaGPRor43 | - | - | - | - | - | - | - | TGCTAGAAATGTGTTTGCTCG | ATCAAATACCAGTCCATGCC |
| AaGPRor44 | +++ | +++ | - | - | - | - | - | TGGAGTTCTGTTTGCTTGGGACAG | TGTTCAATGGTGCGAGTCCACC |
| AaGPRor45 | +++ | +++ | - | - | - | - | - | TCTAACTGTGGGAGGCTTTG | TCATCATAGCGAAGAATTGG |
| AaGPRor46 | - | - | +++ | +++ | - | - | - | AGACCTTCTCATCAAAATGGG | AACATTTCGCTGCTCGG |
| AaGPRor47 | +++ | +++ | +++ | +++ | - | - | +++ | TTGCTTGACCATGATGACC | AAGATGTTACGCTGCTCTGG |
| AaGPRor48 | - | - | - | - | - | - | +++ | TGGCACATGAGATCTACAGC | AATGAGTAAGTGGTTTGGACG |
| AaGPRor49 | - | - | ++ | - | +++ | +++ | - | TCAATGGAGATGAGCTAAGCG | TAATACCGATCTTCCGCTGC |
| AaGPRor50 | +++ | +++ | - | - | - | - | - | TGGGAACGGAACTGATAAGTG | TCATCCTCTGTGATCTTCCC |
| AaGPRor51P | +++ | +++ | - | - | - | - | - | TGGTGGCAAATGCGGTCTATGAGG | TGAAGTAAGACATCGTGGTGCGGAC |
| AaGPRor52 | +++ | +++ | - | - | - | - | - | TGCTGGAGTCTTACTGGTTCTG | ACAATCAGCAGCGTCTCC |
| AaGPRor53 | +++ | +++ | nd | nd | nd | nd | - | TTTCGATGCTGGGAGCTGAACTC | GACGGTCCGCTCTCATTTTCATG |
| AaGPRor54P | +++ | +++ | - | - | - | - | - | ACATGAGGTTTGCTTCCGATCTGC | GGCAGCCATTCGGTTGTACCATT |
| AaGPRor55 | +++ | +++ | +++ | +++ | - | - | - | TGCCGAAAGACGTTTGC | TCCGTAGAACTGCTGCATTATC |
| AaGPRor56 | +++ | +++ | +++ | +++ | - | - | - | ATGTTCGTTCTGCAATATACCG | AACAACAGCAATCGGCTCTCGGTAG |
| AaGPRor57P | +++ | +++ | - | - | - | - | - | ATGGTCGTAATCGCATCG | ATCCGATCTCAGCTTCATG |
| AaGPRor58 | - | - | - | - | - | - | +++ | TCGAAGCCAAACCCCAGGAC | ACAGCCGATGGTTGATGGATGAC |
| AaGPRor59 | +++ | +++ | - | - | - | - | - | ACTTTAAGACAAGCCCTTCGC | AGCACAGAGCATGGATCCC |
| AaGPRor60 | - | - | - | - | - | - | +++ | TTGTAACATGGTGTCGTCGTTG | ATTCCATCAGCTCGATTAGTATGC |
| AaGPRor61 | - | - | - | - | - | - | +++ | TTCACTCCAAAATTCGACAGC | TGCTGTATCTCAAATCGACTCC |
| AaGPRor62 | - | +++ | - | - | - | - | - | TGAACATCGCAGCTGGAG | AGGCCGAAAGACCGATATATTG |
| AaGPRor63 | +++ | +++ | - | - | - | - | - | TCTAGGAACAGCTTTGACGC | TGTTCAGAAGAGCCACGC |
| AaGPRor64P | +++ | +++ | - | - | - | - | - | ATCCGTGTCATGTCTCAGTATG | TGCAGTACAAATTTCAACCG |
| AaGPRor65 | - | - | - | - | - | - | - | ATTTATGCTATCAACTGGCACC | TTGTGATGAGCATCGCC |
| AaGPRor66 | +++ | +++ | - | - | - | - | - | TGACTGGTACGCAACATATG | TCGGTAATAAATGCCATGG |
| AaGPRor67 | - | - | - | - | - | - | - | TGGTCGACATTTATGCTATCAAC | ATTGTGATGAGCATCGCC |
| AaGPRor68P | - | - | - | - | - | - | - | TGTTGTGAATTTTCCGTGG | ACATGATTGGTGTTCTCGATG |
| AaGPRor69 | +++ | +++ | - | - | - | - | - | TGATGCGATGCTTTTGG | AAATTTAACGGAGCAAACCC |
| AaGPRor70 | +++ | +++ | - | - | - | - | - | TCGTCTTCACTGGCGTATTC | TTTGAGCAAATGCCACG |
| AaGPRor71 | +++ | +++ | - | - | - | - | - | GTACTTGGGGACGGAGTTGAGT | GCCGATCCAGAACATCTTTGAGCA |
| AaGPRor72 | - | - | - | - | - | - | - | TTGTGTTCATCGGTTGTGC | TGCTTCCGTGAGTTTGG |
| AaGPRor73P | - | - | - | - | - | - | +++ | CTGCTGTACGGATTCTTGTGCGA | |
| AaGPRor74 | - | - | - | - | - | - | +++ | CAAGCGGTGCGTTGATCGTC | CCGTGTAAGCGAACCACAACACC |
| AaGPRor75 | - | - | - | - | - | - | +++ | GGCATTGGTGTTTCGGGACAA | TGCCCTCATCATCACAATCAGCA |
| AaGPRor76 | - | - | - | - | - | - | - | GCGTTCGACGGCACCAG | CAGCAGCGTGAATGCCGAGT |
| AaGPRor77P | +++ | +++ | - | - | - | - | - | GCGTCATCAGAGGTTGCTGGAA | GATGCCGCCACAAGTGAATCC |
| AaGPRor78 | +++ | +++ | - | - | - | - | +++ | GCAGGCTTGTCCATCGTGTCC | TTCGCTCGTCGCATTCCACA |
| AaGPRor79 | +++ | +++ | - | - | - | - | - | CCTGCTGGTGGTTCAGCTTCG | TCGCCACGTCCCTGATCG |
| AaGPRor80 | +++ | +++ | - | - | - | - | - | GCATTCTGTTCATCGTGGGAGCA | CATTCCACCAACTCTGAACTTGATTCC |
| AaGPRor81 | - | - | - | - | - | - | - | GACGGATTGTCTGGAAGTACGAGGA | TCCGAAATATACTGGATCGCCATAA |
| AaGPRor82P | +++ | +++ | +++ | +++ | - | - | - | CGCATCCGAAACCTACCTGTGTT | TGTCATGTCGTACCGCACAAAGC |
| AaGPRor83P | +++ | +++ | +++ | +++ | - | - | - | CATCCGAAACCTACCTGTGTTGC | ACCGATTTCATCATCGTGGCAAAC |
| AaGPRor84 | +++ | +++ | - | - | - | - | - | TCGGGTTGTTGCTATCGTTGATGA | ACGGAACCAAAAACCCTGTTGAG |
| AaGPRor85 | +++ | +++ | - | - | - | - | - | GACGCTTTGGGACTGTTGGTT | TCCAAATCCCTGCTGAGCTTGT |
| AaGPRor86N | +++ | +++ | - | - | - | - | - | TGCGAGCAGAGCTTACAATAGCAT | TTCTCGTGTGGGGAATAATCGTGA |
| AaGPRor87 | +++ | +++ | - | - | - | - | - | AATCATGCGTCACATTGAGTTGCTT | TGATAAACCCCCGCAGCTAAATTG |
| AaGPRor88 | +++ | +++ | - | - | - | - | - | TGGGGATAACGGTGTTCTTCATCG | TCGGGTGCCAATCTTCATTATCATCA |
| AaGPRor89 | - | - | - | - | - | - | +++ | ATCTGGATGGACGAGCGATATTCAA | GCGTAGGATTTCTGGAGGATCTGC |
| AaGPRor90 | - | - | - | - | - | - | +++ | TCAACAAGGTATTCGGACCAACGA | CGAGGAAAGCGAACAGCGTGTA |
| AaGPRor91N | +++ | +++ | - | - | - | - | - | GATTTTCGCCCAGTAGCCCTGTA | CATCAGGTCCGTCAAATGTTGCTG |
| AaGPRor92 | - | - | - | - | - | - | - | CGTATGTCGCACTTGTGGAGACC | CACCACCAGCATTCAGCGAAAAA |
| AaGPRor93 | +++ | +++ | - | - | - | - | - | TGCTAACGGGATGACTCGCTTTG | GCTGCTGATAGTCGGAATGGTTCTC |
| AaGPRor94 | ++ | +++ | - | - | - | - | - | AAGGATTTTCGTTCGGCTCGGTA | GCAGCATTGACCTGTACGAGCA |
| AaGPRor95 | ++ | +++ | - | - | - | - | - | GCTATTGGGTTCATGGTGGAATGC | TCCGAATGATGTTTTGGCGAATGT |
| AaGPRor96 | - | +++ | - | - | - | - | - | ATGCGTTTGCCGTGACATACTACT | GAGATCAGAAATACTCCAGCACAGC |
| AaGPRor97 | +++ | +++ | - | - | - | - | - | GTTCTGGAGTGCTATTGGTGGTG | CGATCATCAGCGAAGTACGCATCT |
| AaGPRor98 | +++ | +++ | - | - | - | - | - | CGGAGGAATCGGAGGACTGTTT | ACATTTGATTGGTGGTGGGTTGGA |
| AaGPRor99 | ++ | ++ | - | - | - | - | - | ATACGAAGCGGGTCTGGGTAATG | TTCCGCTCGATGTTGTTGATCGTA |
| AaGPRor100 | +++ | +++ | - | - | - | - | - | TGGCAACTACTCCGATATTCCTGGTG | TCGAACAGCCCCAGACATCG |
| AaGPRor101 | - | - | - | - | - | - | - | ATGGGAATCAACGCAACTTCTGGA | GTGTTCAGCAAACGAACGAAAGCA |
| AaGPRor102 | - | - | - | - | - | - | - | AGACGCTTGCTCATCTTGTGCT | TCTGAAACAATCCCAGGCAGTCCA |
| AaGPRor103 | +++ | +++ | - | - | - | - | - | CTCCTGGGAATGCGAATGAAGTTGA | CGAAAATGATGTTCCCGATGGAAGCA |
| AaGPRor104 | +++ | +++ | - | - | - | - | - | GGAATGCGGATGAAACTCGGAATGA | AACAGCCCAAAGCAATCCACTGAA |
| AaGPRor105 | - | +++ | - | - | - | - | - | TGTGCTCGTTGCCTTTATTTCTGC | AATCCGTTGAATCCCATGTTTTGC |
| AaGPRor106 | +++ | +++ | - | - | - | - | - | TGGGAAATCATCAGTCGGGAAATCA | TGAGCGATGAACAGAAATGCTCCA |
| AaGPRor107 | - | - | - | - | - | - | - | CAGGATGTTATCGCACCGTTTCACA | TGCGGTGTTAAGCAATTCAACGAA |
| AaGPRor108P | - | - | - | - | - | - | - | TGTGCCGCTACTTTGCTGTTAGGA | AGTTCATCAGCATTCCTCGTCGTG |
| AaGPRor109 | - | ++ | - | - | - | - | - | TTTCAGCATTGGAACGAGTGGCTA | CATCAGCATCGTGTAGGCAGTGTC |
| AaGPRor110 | +++ | +++ | - | - | - | - | - | GTTGCCCAAGATGTTTGAAATACGC | GTGGGATGTCAGTTCACTTCGGATT |
| AaGPRor111 | +++ | +++ | - | - | - | - | - | GCCTTGGAACATGCTTTTACCTATCC | CGCATTGAGGAAGCTGACAAACG |
| AaGPRor112P | +++ | +++ | - | - | - | - | - | TCGGGAGGTGAGGACTGTTGTG | CGGCTGTAAGGCATCTGTGC |
| AaGPRor113 | +++ | +++ | - | - | - | - | - | TGCATCCGCATTTGCCTACTTG | CACTCCGTTGATGGTGTTGTGC |
| AaGPRor114 | +++ | +++ | - | - | - | - | - | ATTTTAGCCAACCGCTTTCGTTCG | CGGTTGTGGGGCATAAGAATAGCA |
| AaGPRor115 | +++ | +++ | - | - | - | - | - | AAAGTTTTAGCCAACCGCTTTCATTC | CGGTTGTGGGGCATAAGAATAGCA |
| AaGPRor116P | - | +++ | - | - | - | - | - | GCCGCTGTTTCGTTCTTTCTATTGG | TGTTGATGTCGAATATGCCCATGC |
| AaGPRor117 | - | - | - | - | - | - | - | TCCAATTTCCCGTCCCATCATTCT | CGATAGAAAGAACGAAACAGCGGCT |
| AaGPRor118P | - | - | - | - | - | - | - | TGTGTTGGAGTTACCCTCGTTGGA | CATCAAAACGGAGTACGCAACATCG |
| AaGPRor119 | - | - | - | - | - | - | - | TCTTCTGGCGTTGACTGGAATGTTTC | GGGTTACGAAGGTGAATGTGGTGA |
| AaGPRor120P | nd | nd | nd | nd | nd | nd | nd | ||
| AaGPRor121 | - | +++ | - | - | - | - | - | TCCTGTAGCCAAGTTCTGCTCGT | AACAAAGCCGACAAAAGCAATCGT |
| AaGPRor122 | +++ | +++ | - | - | - | - | - | TGGTGCCATCTGGTTGATTCGT | CAGAAACATCAGCACCGAGTAAGCA |
| AaGPRor123 | - | +++ | - | - | - | - | - | AGCATGATATTGGCGGACTGTTGAA | TGTTCGCATTGCCACAAAGTCAGA |
| AaGPRor124 | +++ | +++ | - | - | - | - | - | TGTCGTCGCTTGGAACTGGAAT | GCTGTGACGGGCGTTAATCCA |
| AaGPRor125N | +++ | +++ | - | - | - | - | - | CTGGATGCCGCTGATTTGGT | CATTCCCAAAAGTGGACGGATCAA |
| AaGPRor126C | +++ | +++ | - | - | - | - | - | ATGAAAGCGATTGTTAGCTTCTGGAA | GCGACTAATGCCAAAGGTTTCCAT |
| AaGPRor127 | nd | nd | nd | nd | nd | nd | nd | ||
| AaGPRor128P | nd | nd | nd | nd | nd | nd | nd | ||
| AaGPRor129P | nd | nd | nd | nd | nd | nd | nd | ||
| AaGPRor130C | - | - | - | - | - | - | - | GGACCTGCTGATGATTGCTTTTGG | TTTTGCGGTAACACCTTCCCACAG |
| AaGPRor131P | nd | nd | nd | nd | nd | nd | nd | ||
| Summary | 75/123 | 84/123 | 13/123 | 13/123 | 3/123 | 3/123 | 24/123 | ||