Figure 2.

Inhibition of in vitro capillary-like structure formation by flavonoids in HBMEC. In order to assess the potential anti-angiogenic properties of flavonoids, in vitro tubulogenesis assay was performed with HBMEC seeded on top of Matrigel as described in the Materials and Methods section. A) Molecules (30 μM) were added 30 minutes after seeding of the cells on top of Matrigel. Structure formation was monitored after 18 hours. Representative phase contrast pictures were taken. B) The extent of three-dimensional capillary-like structure formation (tubulogenesis) was assessed as described in the Materials and Methods section. The length of the tube network was quantitated using Northern Eclipse software. Data are representative of three independent experiments.

Note: *Significant at P < 0.05.

Abbreviation: HBMEC, human brain microvascular endothelial cells.