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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2011 Feb 24;31(5):1185–1191. doi: 10.1161/ATVBAHA.111.224436

Figure 1. The transcription factor ELF4 is expressed in endothelial cells.

Figure 1

(A) Nuclear expression of ELF4 was determined by immunofluorescence in HUVEC cells using anti-ELF4 (red) and phalloidin (green). Magnification: 40x. (B) Immunohistochemistry in E14.5 embryos using an anti-ELF4 antibody. Arrows indicate positive endothelial cells in endocardium (left) and a blood vessel (right). Magnification: 20x. (C) Immunofluorescence of yolk sac (E9.4) using smooth muscle actin (SMA, green), anti-CD31 (blue), and anti-ELF4 (red). (D) Immunofluorescence of murine hematopoietic (CD45+) and non-hematopoietic endothelial cells (CD45− CD31+). Arrows indicate CD31 surface expression. Magnification: 40x.