FIG. 4.

Adsorption of rXyn5(120k) (A) and rXyn5(90k) (B) to a crystalline cellulose, Avicel PH-101. Ten micrograms of rXyn5(120k) or rXyn5(90k) was incubated with Avicel PH-101 (10 to 40 mg) at 4°C for 30 min in 300 μl of 50 mM potassium phosphate buffer (pH 7.0). After centrifugation, the supernatants (S) obtained were stored at 0°C and the precipitates (P) were washed and suspended in the same buffer. Portions of the S and P fractions were analyzed by Western immunoblotting. M, molecular markers. The solid and open arrowheads indicate the positions of rXyn5(120k) and rXyn5(90k), respectively.