Figure 5. Changes in plastid ultrastructure in relation to the accumulation of chlorophyll and Rubisco in the silenced plants.

Germinated seeds (line 20 and WT) were grown in growth chambers at either 30°C (A) or 22°C (B) and under 80 µmol·m⁻² s⁻¹ light intensity for various times (3, 6, 10d). The newest leaf (3, 6d) or the second leaf from the top (10d) was sampled for the observation of ultrastructure of plastids (A and B) and determination of chlorophyll and Rubisco content (C and D). The ultrastructure was observed by a Philip Fei-Tecnai 12 transmission electron microscope. Bars = 0.5 µm. The data are means ±SD of 3 replicates and representative of at least three independent experiments.