Table 3. COBRA analysis of 17 selected genes in primary tumor and adjacent liver.
| Genes | Primary Tumor | Adjacent Liver | P Value |
| WNK2 | 3/5 | 0/5 | 0.038 |
| FOXE3 | 5/5 | 4/5 | 0.292 |
| GUCY1A2 | 2/5 | 3/5 | 0.294 |
| EMILIN2 | 3/5 | 0/5 | 0.038 |
| TRIM58 | 3/5 | 0/5 | 0.038 |
| ADCY5 | 4/5 | 2/5 | 0.197 |
| OVAL1 | 3/5 | 2/5 | 0.527 |
| ADAM8 | 5/5 | 4/5 | 0.292 |
| GRASP | 3/5 | 0/5 | 0.038 |
| UTF1 | 4/5 | 4/5 | 1 |
| BOLL | 2/5 | 5/5 | 0.079 |
| TM6SF1 | 4/5 | 0/5 | 0.01 |
| KIF17 | 0/5 | 0/5 | 1 |
| EPHA4 | 1/5 | 0/5 | 0.292 |
| ATP8A2 | 3/5 | 3/5 | 1 |
| HIST1H4F | 3/5 | 0/5 | 0.038 |
| TLX3 | 3/5 | 0/5 | 0.038 |
The genes selected from Illumina Infinium methylation assay were validated in COBRA with five pairs primary tumor and adjacent liver, ten genes shared the same methylation rate in primary and adjacent liver by our COBRA primers were excluded.