Table 4. COBRA analysis of 7 selected genes in primary tumor and adjacent liver.
| Genes | Primary Tumor | Adjacent Liver | P Value |
| WNK2 | 7/10 | 2/10 | 0.025 |
| EMILIN2 | 7/10 | 0/10 | 0.001 |
| TRIM58 | 7/10 | 1/10 | 0.006 |
| GRASP | 7/10 | 2/10 | 0.025 |
| TM6SF1 | 8/10 | 2/10 | 0.005 |
| HIST1H4F | 7/10 | 0/10 | 0.001 |
| TLX3 | 6/10 | 1/10 | 0.02 |
Seven genes confirmed frequent hypermethylation in primary tumor tissues (p<0.05) were selected for further analysis in ten pairs of primary tumor and adjacent liver tissues, frequent hypermethylation (P<0.05) were observed in these genes.