Figure 3. The effect of PTHrP neutralization on cell proliferation.

Cells were treated with anti-PTHrP antibodies and assayed for cell growth using both WST-1 assay (A–D) and CV staining (E) over a two-day period. Total cell number was evaluated by measuring absorbance at 450 nm and 570 nm respectively. Cells assayed were A) CRL1932, B) hFOB, C & E) GCT stromal cells from primary bone and D) from a lung metastasis. Results are means ± SEM of triplicate independent experiments after normalized to the background control.