Table 1. Human primer sequences special designed for real-time RT-PCR amplification.

Gene	Forward/Reverse	Primer sequence	Accession #	Size of product (bp)	Melting temperature (°C)
PTHrP	F	5′ GGA GAC TGG TTC AGC AGT GG 3′	NM_002820	133	57.0
	R	5′ TTG TCA TGG AGG AGC TGA TG 3′	(all 4 variants)		54.0
GAPDH	F	5′ CAT GAG AAG TAT GAC AAC AGC CT 3′	NM_002046	113	55.0
	R	5′ AGT CCT TCC ACG ATA CCA AAG T 3′			56.0
Caspase-3	F	5′ CAT GGA AGC GAA TCA ATG GAC T 3′	NM_004346	139	56.0
	R	5′ CTG TAC CAG ACC GAG ATG TCA 3′			56.0
Caspase-9	F	5′ AGG ATT TGG TGA TGT CGG TG 3′	NM_001229	119	55.0
	R	5′ CAC GGC AGA AGT TCA CAT TG 3′			55.0