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. 2011 May 23;6(5):e20291. doi: 10.1371/journal.pone.0020291

Figure 2. HIF-1α is a target of miR-22.

Figure 2

(A) Alteration of miR-22 expression by transfection. HCT116 cells were transfected with pre-miR-22 or anti-sense-miR-22 or control oligonucleotides, and levels of miR-22 were measured by qPCR. (B) Over-expression of miR-22 inhibits HIF-1α expression. HCT116 cells were transfected with control oligonucleotides or pre-miR-22, and then exposed to normoxia or hypoxia for 16 h. Cell lysates were immunoblotted for HIF-1α. Hypoxia induces HIF-1α, but miR-22 suppresses HIF-1α. (C) Two colon cancer cell lines, HCT116 (two upper panels) and HT29 (two bottom panels), were transfected with pre-miR-22 or anti-sense-miR-22, exposed to hypoxia, and cell lysates were immunoblotted for HIF-1α. (D) Quantification by densitometry of immunoblotting for HIF-1α in HCT116 cells transfected as above (n = 3± S.D.).