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. 2011 May 23;6(5):e20291. doi: 10.1371/journal.pone.0020291

Figure 3. MiR-22 regulates HIF-1α by inhibiting its translation.

Figure 3

(A) MiR-22 does not affect HIF-1α mRNA. HCT116 cells were transfected with Pre-miR-22 or anti-sense-miR-22 or control. Total RNA was harvested and analyzed for HIF-1α mRNA by qPCR (n = 3± S.D.). (B) Human HIF-1α 3′UTR contains a binding site for miR-22. (C) MiR-22 represses transactivation of HIF-1α 3′UTR. HCT116 cells were transfected with a luciferase reporter vector (MiRreport) containing the 3′ UTR of HIF-1α (left panel) or a mutated 3′UTR of HIF-1α (right panel) and with pre-miR-22 or pre-miR-control. Cells were harvested and luciferase activity was measured (n = 3± S.D.).