Table 2. Plasmids constructed and used in this study.

Plasmids	Relevant characteristic(s)	Source of reference(s)
pMSD8	Apr; pFN476 derivative containing E. coli accBCDA genes; T7 promoter	[12]
pMSD15	Cmr; pACYA184 derivative containing E. coli 'tesA gene (without leading sequence); PBAD promoter	[12]
pXL67	Apr; pET28b derivative containing Acinetobacter baylyi ADP1 atfA gene; T7 promoter	Gift from Khosla
pXL72	Kmr; pCR-Blunt vector derivative containing E. coli fadD gene	Gift from Khosla
pKD46	Apr; Vector expressing the Red genes (γ, β, and exo) from phage λ under the arabinose-inducible araB promoter, oriR101, repA101(Ts)	[13]
pKD4	Apr Kmr; Template plasmid for kan flanked by FRT sequences, oriR6K	[13]
pCP20	Apr Cmr; Helper plasmid, FLP λ cI857 λ PR repA(Ts)	[14]
pXT9	Apr; pET28b derivative containing atfA-fadD genes; T7 promoter	This study
pXT10	Apr; pET28b derivative containing pdc-adhB genes; T7 promoter	This study
pXT11	Kmr; pET28b derivative containing atfA-fadD-pdc-adhB genes; T7 promoter	This study