Figure 8. Site-directed mutagenesis of overlapping motifs in CNE2.

(A) Wild-type and mutant sequences of overlapping motifs in CNE2. (B) lacZ expression although still detectable in neural tube and dorsal root ganglia, is greatly reduced especially in the anterior neural tube. (C, D) Faint lacZ expression in neural tube is present in two different embryos, and visible under higher magnification in (E, F). Yellow scale bar denotes 1 mm in length. Red scale bar denotes 0.2 mm in length.