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. Author manuscript; available in PMC: 2012 Feb 5.
Published in final edited form as: Virology. 2010 Dec 3;410(1):170–180. doi: 10.1016/j.virol.2010.11.006

Fig. 3.

Fig. 3

Production of VLPs and prM-E heterodimerization of the MH domain mutants of DENV1 and DENV2 prM/E constructs. (A, B) At 48 h post-transfection, 293T cell lysates and pellets derived from culture supernatants of WT or mutants were subjected to Western blot analysis using human serum of a dengue case (Wang et al., 2006). The long exposure gels for DENV1 prM bands in pellets were shown below. (C) Transfected 293T cells were labeled with 35S-Met, immunoprecipitated with anti-E Mab FL0232 and subjected to SDS-12% PAGE. The size of molecular weight markers is shown in kDa. One representative experiment of two is shown.