Table 1.

Antibodies used in the study

Primary antibody	Source and catalog number	Immunogen and preparation	Specificity	Cell Types Stained
Chicken α–GFP	AbCam ab13970	Recombinant full-length protein. This anti-GFP antibody recognizes the enhanced form of GFP, and all of the fluorescent proteins made by this algae, including yellow FP.	Antibody stains various transgenic reporter mice bearing a GFP transgene, it does not stain wild type littermates.	GFP-labeled cells from ΔSox2-eGFP heterozygous mice, Neurog1-eGFP BAC transgenic mice, or constitutive GFP- expressing mice
Rabbit α–GFP	AbCam ab6556	Highly purified recombinant full length GFP made in Escherichia coli	Antibody stains various transgenic reporter mice bearing a GFP transgene, it does not stain wild type littermates.	GFP-labeled cells from ΔSox2-eGFP heterozygous mice, Neurog1-eGFP BAC transgenic mice, or constitutive GFP- expressing mice
Goat α–Sox2	Santa Cruz, sc-17320	Amino acids 277-293 of human Sox2 affinity- purified. YLPGAEVPEPAAPSRL H	Monospecific, reacting with a 34 kDa band in WB of mouse and human ES cell lysates	Sustentacular cells, HBCs and several subsets of GBCs
Rabbit α–Sox9	Chemicon (Millipore) AB5535	Synthetic peptide from human Sox9, affinity purified	Recognizes 60-65 kDa band corresponding to Sox9 on Western blot of fetal mouse brain lysate	Bowman's gland and duct cells
Rat α–E- cadherin	Sigma U 3254	Rat embryonal carcinoma line PCC4 AZA R1 was used as immunogen. The antibody is a monoclonal IgG1 provided in rat ascites fluid from the DECMA-1 hybridoma line. Monoclonal Anti-E- Cadherin was selected against the mouse E-Cadherin.	It blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre- implantation embryos. The antibody disrupts confluent monolayers of MDCK epithelial cells. (Sigma Datasheet)	Sustentacular cells (weakly) and Bowman's gland and duct cells (strongly)
Goat α–CD54	R&D systems, AF583	Recombinant extracellular domain from rat, affinity- purified (Accession Q00238, Gln 28 - Thr 493)	Blocks adhesion to ICAM-1, WB confirmed	HBCs
Rabbit α–PGP9.5	Cedarlane/Ultraclone RA95101	Human PGP9.5 protein purified from pathogen free human brain	Cross reacts with all mammalian species tested. {Wilson, 1988 #938}	Immature and mature OSNs
Rabbit α–CK 18	Abcam 52948	A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 18	Detects a band of approximately 48 kDa on WB. (Abcam datasheet)	Sustentacular cells, Bowman's gland and duct cells
Mouse α–CK14	Vector #VP-C410	Synthetic peptide corresponding to C- terminus region of human keratin 14 (GKVVSTHEQVLRTK N) conjugated to thryoglobulin.	Selectively labels basal cells in skin, with a cytoplasmic distribution (Vector Labs Datasheet)	HBCs
Rabbit α–CK14	LabVision #RB- 9020-P1	A synthetic peptide of 15 amino acid residues from the C-terminus of human keratin 14.	Identical staining as above Mouse α–CK14. Stains A431 cells and skin or squamous cell carcinoma (Lab vision data sheet)	HBCs
Mouse α–Ki67	BD-Biosciences, 556003 (clone B56)	22 aa Ki-67 repeat motif (APKEKAQPLEDLASF QELSQ)	Reacts with 345/395 kDa doublet on WB corresponding to Ki-67 Ag; B56 binding to cells blocked by clone MIB 1, the canonical α–Ki67 antibody	Proliferating cells
Rat α–NCAM	Abcam ab19782 (H28-123)	Glycoprotein fraction from neonatal mouse brain	Recognizes at the neural cell surface a triplet of glycoproteins neural BSP2, which is identical to NCAM (Abcam datasheet)	Immature and mature OSNs
Mouse α– neuron specific tubulin	Covance MMS-435P (TuJl)	Microtubules derived from rat brain.	Highly reactive to neuron specific Class III ß-tubulin (ßIII). Does not identify ß-tubulin found in glial cells.	Immature OSNs
Rabbit α–PCNA	Abcam ab2426-1	Synthetic peptide: DMGHLKYYLAPKIED EEGS, corresponding to C terminal amino acids 243-261 of Human PCNA.	Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa) which can be blocked with PCNA peptide (ab2427) (Abcam datasheet)	Proliferating cells
α–vimentin	Santa Cruz, sc-7557	A synthetic peptide at the C-terminus of human vimentin	Detects a band of approximately 57 kDa which can be blocked with sc-7557 P (Santa Cruz datasheet)	LPimm cells
α–Thy1.2	eBioscience, 14-0902 (clone 53-2.1)	Mouse thymus/spleen	Reacts with mouse CD90.2 (also known as Thy1.2, a GPI-linked membrane molecule; eBioscience datasheet)	LP-derived fibroblasts
α–PECAM	BD Bioscience, 550274 (clone MEC13.3)	129/Sv mouse-derived endothelioma cell line tEnd.1	Stains endothelial cells blood vessels in spleen, lung, heart and thymus (BD-Bioscience data sheet)	LP-derived endothelial cells