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. Author manuscript; available in PMC: 2012 May 20.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Apr 21;408(4):630–634. doi: 10.1016/j.bbrc.2011.04.075

Figure 1.

Figure 1

Attenuation of Thrombin-induced Decreases in TEER by Inhibition of HDAC6. A) HPAECs were exposed to thrombin in the presence or absence of tubacin. TEER was measured at the indicated time points post-exposure. **, a comparison between the control and the thrombin treated groups; *, a comparison between the thrombin+DMSO and the thrombin+tubacin groups. B) An experiment that is similar to part A except that MC1575 replaced tubacin. C) The culture conditions were identical to A. Protein levels of Ac-α-tubulin and α-tubulin were determined using immunoblots. D) An experiment that is similar to part A except that MC1575 replaced tubacin. “S” refers to DMSO, the solvent for MC1575. E) Total protein was extracted from mouse lungs exposed to LPS with or without tubacin. The protein levels of Ac-α-tubulin and α-tubulin were determined. F) LPS-exposed mouse lungs were dissected and wet/dry weight ratios were measured as described in the Methods Section. Means and standard errors were obtained from 7 mice per group. *, **, and *** denote a P value < 0.05, 0.01, and 0.001, respectively.