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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Exp Neurol. 2011 Apr 2;229(2):471–483. doi: 10.1016/j.expneurol.2011.03.018

Figure 1. KCNQ2/3 channel expression and M-currents in mouse ES cell-derived neurons and hippocampal neurons.

Figure 1

Expression and functional activity of KCNQ2 and KCNQ3 channels were tested in mouse stem-derived neurons and hippocampal neurons (12–15 DIV). A. Western blotting demonstrated that KCNQ2 and KCNQ3 are expressed during differentiation in mouse stem cell-derived neurons or hippocampal neurons. Representative of N ≥ 3 assays. B. Whole cell recording showed M-currents are present in neurally differentiating cells 3 and 5 days after neuronal induction from stem cells or in hippocampal neurons of 14 DIV. Representative of ≥ 20 cells in each recording. C. Bath application (10 min) of 10 μM XE991, a selective KCNQ channel inhibitor, blocked the M-currents recorded in hippocampal neurons. N ≥ 5 cells in each group.