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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Exp Neurol. 2011 Apr 2;229(2):471–483. doi: 10.1016/j.expneurol.2011.03.018

Figure 11. Different effects of KCNQ2/3 channel activities on endocytosis and exocytosis in mouse stem cell-derived neurons.

Figure 11

Fluorescent styryl-dye FM4-64 was used to monitor endocytosis and exocytosis in ES cell-derived neurons after 5 or 12 days differentiation. A. Schematic diagram showed the protocol of FM4-64 staining. B to D. Fluorescent microscopy images and quantification of the intensities of FM4-64 after treatment in XE991 or linopirdine for 5 (C) or 12 (D) days. Both XE991 and linopirdine reduced endocytosis at the two time points, while they equivalently upregulated exocytosis. Scale bar=50 μm. Mean ± S.E. M. N ≥ 3 independent experiments. * P < 0.05 vs. vehicle controls.