Skip to main content
. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Exp Neurol. 2011 Apr 2;229(2):471–483. doi: 10.1016/j.expneurol.2011.03.018

Figure 2. Association of KCNQ2 and KCNQ3 proteins with synaptophysin.

Figure 2

Immunoprecipitation, Western blotting and immunostaining were applied to examine possible association of KCNQ2/3 channels with the presynaptic protein synaptophysin. A. Proteins were extracted from the adult mouse brain and used in immunoprecipitation assays against synaptophysin. Western blotting was then performed to detect KCNQ2, KCNQ3 or synaptophysin expression as an indication of the formation of protein complex between KCNQ2/KCNQ3 with synaptophysin. Both KCNQ2 and KCNQ3 were presented in the protein complex with synaptophysin. N = 3 assays. B and C. Immunostaining showed that KCNQ2 was also co-localized with synaptophysin in mouse stem cell-derived neurons (B) and hippocampal neurons (12–15 DIV) (C).