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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Exp Neurol. 2011 Apr 2;229(2):471–483. doi: 10.1016/j.expneurol.2011.03.018

Figure 3. Chronic blocking KCNQ2/3 channels did not affect cell viability.

Figure 3

As a control experiment, we evaluated cell viability of hippocampal neurons under our experimental culture conditions. A. Phase contract images of hippocampal cultures 7 days after wash control, XE991 (10 μM) and linopirdine (50 μM). B. Fluorescent images of Hoechst 33342 and TUNEL staining of hippocampal neurons 7–14 days in XE991 (10 μM) treatment. C and D. Quantification of data from the experiments in B. Chronic exposure to XE991 and linopirdine did not cause cell death in hippocampal cultures. N ≥ 3 assays.