Figure 9. Effects of blocking KCNQ2/3 channels on neuronal differentiation/maturation of ES cell-derived neurons and hippocampal neurons.
Bar graphs summarized Western blotting analyses of GluR1 and GAD67 expression in cells treated with KCNQ2/3 channel inhibitors. A. Mouse ES cell-derived neurons were treated with a KCNQ channel blocker for 5 days, the treatment caused no alteration in the expression of GluR1 and GAD67 in these cells. B and C. Hippocampal neurons were subjected to 7 or 14 days exposure to XE991 (10 μM) or linopirdine (30 μM), respectively. Although the 7 day treatment did not show significant effect, 14 day treatment with XE991 or linopirdine selectively reduced GAD67 expression compare with control cells. Mean ± S.E. M. N ≥ 3 independent experiments. * P < 0.05 vs. vehicle controls.