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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Biomaterials. 2011 Apr 13;32(22):5065–5076. doi: 10.1016/j.biomaterials.2011.03.053

Figure 2. Enhanced osteoblast differentiation from mouse iPS cells by the transfection of SATB2 gene.

Figure 2

(A) Matrix mineralization in the cells was detected by Alizarin Red S and von Kossa assay in Vector-, SATB2- transduced cells and untransduced control cells after 14 days culture (a-h, 100×) with or without osteogenic supplements (OS). Quantitative analysis of calcium nodules (B) present and alkaline phosphatase (ALP) activity (C) in the four groups. A significant increase is seen in the SATB2 group when compared with the Vector and untransduced control groups. (D) Total RNA was isolated, and real-time quantitative PCR (RT-qPCR) was carried out using primers for SATB2, OSX, Runx2, BSP, OCN, HoxA2 and GAPDH. iPS w/o OS: non-treated iPS-EBs; iPS + OS: iPS-EBs with OS; iPS-Vector + OS: iPS-EBs with OS plus Vector; iPS-SATB2 + OS: iPS-EBs with OS plus SATB2; w/o: without. These data are expressed as the mean ± S.D. (n=3). ** p < 0.01, *** p < 0.001 compared with non-transduced or Vector -transduced iPS-EB cells. NS: no significant.