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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Biomaterials. 2011 Apr 13;32(22):5065–5076. doi: 10.1016/j.biomaterials.2011.03.053

Figure 5. Analysis of new bone formation in mice at 5 weeks post-operation.

Figure 5

(A) (a) Morphometric analysis (BV/TV) of new bone formation, (b) volume of newly regenerated bone; (c) local bone mineral density (BMD) analysis by microCT of five groups at 5 weeks post-operation. Quantitative micro-CT analysis revealed that implantation of SATB2 transduced iPSCs/SS constructs achieved the most amount of new bone formation, volume of newly regenerated bone and the highest local BMD than other four groups, ***p<0.001, NS: no significant. (B) Total RNA was isolated from newly formed tissue, and RT-qPCR was carried out using primers for Runx2, BSP, OCN, HoxA2 and GAPDH.