Fig. 4.

Comparison of TH+ immunoreactive protein levels in the ventral midbrain (VMB) of WT versus NL mice. The VMB was dissected by performing 2 coronal cuts, one immediately anterior to the superior colliculus and a second immediately posterior to the inferior colliculus which was directed to then pass ventrally through the margin between the midbrain and the pons. A transverse cut just superior to the visible cerebral peduncles was used to separate the VMB from the dorsal midbrain. The tissues were homogenized and western blots were subsequently performed as outlined in the caption to Figure 2 except that the TH+ immunoreactive protein was normalized to beta actin. A - p<0.02 from WT