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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Neurochem Int. 2011 Feb 18;58(8):872–879. doi: 10.1016/j.neuint.2011.02.014

Fig. 2.

Fig. 2

Changes in expression of VEGF, Ang-1, and Ang-2 after MCAO. (A) (Top) RT-PCR of VEGF, Ang-1, and Ang-2 mRNA at the indicated time points after MCAO; (Bottom) bar graph showing mRNA expression relative to that of β-actin. (B) (Top) Western blot of VEGF, Ang-1, and Ang-2 at the indicated time points after MCAO; (Bottom) bar graph showing protein expression relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Data are presented as means ± S.D.; n = 6/group; #P < 0.05, *P < 0.01. (C) Immunohistochemical staining revealed VEGF and Ang-2 immunoreactivity in neuron-like and glial-like cells in the ischemic boundary area after MCAO. Ang-1 immunostaining was especially weak at 1 day after ischemia. Scale bar = 20 μm.