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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Neurochem Int. 2011 Feb 18;58(8):872–879. doi: 10.1016/j.neuint.2011.02.014

Fig. 4.

Fig. 4

Effect of MCAO on brain water content and ZO-1 expression. (A) Brain water content was calculated as the weight difference between wet and dry samples. Data are means ± S.D.; n = 7 per group; *P < 0.01 compared with sham group. (B) Northern blot of ZO-1 mRNA at the indicated time points after MCAO. β-actin was used as a loading control. (C) Western blot of ZO-1 protein after MCAO. GAPDH was used as a loading control. Data are presented as means ± S.D.; n = 6 per group, #P < 0.05, *P < 0.01 compared with the sham group. (D) ZO-1 immunoreactivity was observed around the vessels, especially at sites of endothelial cell–cell contact in control brain. ZO-1 was weakly expressed at 1 day after MCAO, and strongly expressed at 7 days. Scale bar = 50 μm.