Table 3.

Hepatocyte cultures incubated with volatile CPs over 3 h

	CC	TMT	β-IO	DHA
CPs in cell culture prior to incubation (N = 3)
Mean ± 95% CI (μg/ml)a	1.53 ± 0.09	1.77 ± 0.01	1.85 ± 0.11	2.00 ± 0.61
CPs in cell culture after 3 h incubation
Mean ± 95% CI (μg/ml)a
Dish 1 (N = 3)b	0.32 ± 0.02	ndc	0.14 ± 0.01	0.94 ± 0.30
Dish 2 (N = 3)	0.31 ± 0.02	nd	0.13 ± 0.01	0.94 ± 0.30
Dish 3 (N = 3)	0.36 ± 0.02	nd	0.14 ± 0.01	0.83 ± 0.26
Dish 4 (N = 3)	0.35 ± 0.02	nd	0.11 ± 0.01	0.82 ± 0.26
Remaining CPs (%) in medium after 3 hd	18.0	nd	5.0	47.4
Cellular uptake/adhesion relative to initial concentration (%)e	31.6	68.6	59.9	36.2

^a95% confidence interval calculated from linear regression derived from SPE GC-MS

^b N referring to replicate injections of the culture eluate derived from individual dish

^cTMT concentration was smaller than LOD given in Table 1

^dCPs recovered in cultures after 3 h incubation relative to initially applied concentrations calculated from corrected peak areas

^eCP reduction corrected by evaporative losses, i.e., CPs recovered in cell-deficient media due to evaporation over 3 h incubation minus CPs recovered in the presence of hepatocytes under identical incubation conditions and concentrations