Table 1.
Antibodies: source and use.
Primary antibodies | Secondary antibodies |
---|---|
(dilution, if not stated otherwise, 1 : 400) | |
Mouse monoclonal anti-hNANOG (N3038, Sigma) 1 : 75 | Goat anti-mouse IgG-Alexa Fluor 488 (A31619, Invitrogen) or 594 (A31623, Invitrogen) |
Rabbit polyclonal anti-hOCT4 (ab19857, Abcam) 1 : 200 | Goat anti-rabbit-IgG-Alexa Fluor 594 (A31631, Invitrogen) |
Goat polyclonal anti-hOCT3/4 (N-20) (sc-8630X, Santa Cruz) 1 : 400 | Rabbit anti-goat IgG-Cy3 (C2821, Sigma) 1 : 500 |
Rabbit polyclonal anti-hP21 C-19 (sc-397, Santa Cruz) IF 1 : 50 | Goat anti-rabbit-IgG- .Alexa Fluor 594 (A31631, Invitrogen) |
Rabbit polyclonal anti-hP16 N-20 (sc-467, Santa Cruz) IF 1 : 50 | Goat anti-rabbit-IgG- .Alexa Fluor 594 (A31631, Invitrogen) |
Mouse monoclonal anti-hPML (sc-966, Santa Cruz) 1 : 150 | Goat anti-mouseIgG-Alexa Fluor 488 (A31627, Invitrogen) |
Rabbit polyclonal anti-hAURORA B (ab2254, Abcam) 1 : 300 | Goat anti-rabbit-IgG- .Alexa Fluor 594 (A31631, Invitrogen) |
Rabbit polyclonal anti-h-γH2AX (4411-PC-100, R&D Systems) 1 : 200 | Goat anti-rabbit-IgG- .Alexa Fluor 594 (A31631, Invitrogen) |