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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Appl Toxicol. 2010 Jul;30(5):416–430. doi: 10.1002/jat.1513

Figure 7. Keratin immunofluorescent staining with DAPI counter stain in arsenite-transformed isolates.

Figure 7

Keratin 6 staining is shown for (A) UROtsa parent; (B) As #1; (C) As #2; (D) As #3; (E) As #4; (F) As #5; (G) As #6. Keratin 6 positive staining was observed in all isolates, however, keratin 6 was found to organize into strongly stained intermediate filaments in only a subset of the isolates As #1 (B), As #3 (D), and As #4 (E). Strongly stained keratin 16 intermediate filaments are shown for the As #4 isolate (H) and strongly stained keratin 17 filaments are shown for the As #6 isolate (I). Controls included staining using the secondary-antibody only and were found to be negative for all isolates and for both secondary antibody conditions (not shown). Bar = 20 μm.