Figure 4.

The effect of WF10 on the transcription of NK cell genes. Freshly isolated NK cells were incubated with WF10 (at a chlorite concentration of 200 μM) for 0, 3, or 18 h and mRNA was isolated. The indicated transcripts were quantified by quantitative RT-PCR. The number of transcripts of individual genes in each sample was normalised using the number of transcripts of the house-keeping genes β-actin and cyclophilin b. The number of transcripts in the WF10-treated sample was compared with the number of transcripts in the control sample using the formula log  2 (transcripts WF10 sample/transcripts control sample). Average values and standard deviation from 5 independent experiments with different blood donors are shown.