Table 4.

Binding Affinities at hD2R, hD3R and Chimeric Receptors for Compound 8d

		IABN	Compound 8d
	D2 Wild type	0.03 ± 0.004	9,163 ± 3731
	Chimera A	0.05 ± 0.009	2,369 ± 945
	Chimera B	0.02 ± 0.004	13.9 ± 1.8
	Chimera C	0.02 ± 0.006	20.8 ± 3.0
	Chimera D	0.01 ± 0.003	11.5 ± 2.1
	Chimera E	0.03 ± 0.001	1.7 ± 0.1
	Chimera F	0.03 ± 0.011	2.9 ± 0.3
	D2/D3 E2 loop	0.08 ± 0.007	245 ± 37
	D3/D2 E2 loop	0.03 ± 0.004	31.5 ± 3.0
	D3 Wild type	0.04 ± 0.003	5.4 ± 0.6

All of the dissociation constants are expressed as nM and are the mean± S.E.M. For IABN Kd values were obtained by Scatchard analysis of direct binding studies and values for the other compounds are Ki values obtained from competitive radioligand binding analysis. The number of independent experiments performed to obtain the mean values is n ≥ 3 for the chimeric receptors. For the binding of ¹²⁵I-IABN to D2R wild type n = 10 and for the D3R wild type n = 13. For the binding of 8d to D2R wild type n = 7 and for the D3R wild type n = 5.