Figure 5. Developmental RT-PCR analysis of tbx22-1 and tbx22-2 mRNAs.
A) The developmental expression of zebrafish tbx22-1 and tbx22-2 RT-PCR products, 402 and 515 bp respectively, size fractionated in a non-denaturing TAE agarose gel. B) tbx22-1 and tbx22-2 RT-PCR products size fractionated in a denaturing, alkaline agarose gel. The non-denatured, heteroduplex, high molecular weight product present in Panel A is eliminated in the alkaline denaturing gel. C) β-actin control RT-PCR products.