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. 2010 Oct 14;2011:834948. doi: 10.1155/2011/834948

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Copyright © 2011 Sébastien Cagnol et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) Raf-1 activation inhibits caspase-9 cleavage upon serum withdrawal. CCL39-ΔRaf-1:ER cells treated with or without 4-HT were deprived of serum for the indicated time periods. The cell lysates were analyzed by immunoblotting for caspase-9, phospho-MAPK1,3, and total MAPK1. (b) Raf-1 activation inhibits caspase activity CCL39-ΔRaf-1:ER-cells treated or not with 4-HT were deprived of serum for 14 hours as indicated. sts: the effect of 0.3 mM staurosporin is shown as a comparison. Caspase 9 activity was measured using the peptide LEHD-pNA as a substrate and caspase 3 activity using DEVD-pNA. The mean values ± SEM were calculated from at least two separate experiments performed in triplicate.