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. 2010 Oct 14;2011:834948. doi: 10.1155/2011/834948

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Copyright © 2011 Sébastien Cagnol et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) Raf-1-mediated protection of caspase 9 cleavage requires protein synthesis. CCL39-ΔRaf-1:ER cells were deprived of serum for a total time of 24 hours in the presence or absence of 1 μM 4-HT. The protein synthesis inhibitor emetine (20 μM) was added at the indicated time point. The cell lysates were analyzed by immunoblotting for caspase-9, phospho-MAPK1,3, total MAPK1 and XIAP. (b) and (c) time course of Raf-1-mediated caspase 9 inhibition. (b) CCL39-ΔRaf-1:ER cells were deprived of serum for a total time of 24 hours in the presence of 50 nM 4-HT. 10 μM U0126 was added at the indicated time after serum withdrawal. (c) Cells were deprived of serum for 24 h; 4-HT (1 μM) was added at the indicated time after serum withdrawal. The cell lysates were analyzed by immunoblotting for caspase-9.