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Identification of mutant TR isoform-associated proteins from HeLa cells by tandem-affinity chromatography and proteomic analysis. A, Nuclear extracts prepared from control (lane 1), FH-αPV (lane 2), or FH-βPV (lane 3) were purified by tandem affinity chromatography as described in Materials and Methods. B, After peptide sequencing and proteomic analysis, the number of nuclear proteins associated with TR mutant isoforms is shown.
