Table 1.
Proteins identified from tandem-affinity chromatography and proteomic analysis
| Band number | Symbol | Protein name | Protein coverage (%) | Accession no. | Bound to |
|---|---|---|---|---|---|
| 1 | SPTAN1 | Spectrin, α, nonerythrocytic 1 (α-fodrin) isoform 2 | 39.04 | NP_003118.2 | α1PV |
| SPTBN1 | Spectrin, β, nonerythrocytic 1, isoform | 25.20 | EAX00138.1 | α1PV | |
| CHD4 | Chromodomain-helicase-DNA-binding Protein 4 | 9.94 | Q14839.1 | α1PV | |
| FLNA | Filamin A, α isoform 2 | 7.97 | NP_001104026.1 | α1PV | |
| RIF1 | RAP1 interacting factor 1 | 1.54 | NP_060621.3 | α1PV | |
| 3 | KIF11 | Kinesin family member 11 | 8.05 | NP_004514.2 | α1PV |
| NCoR1 | Nuclear receptor co-repressor 1 | 2.39 | BAA75814.1 | α1PV | |
| SF3B3 | Splicing factor 3b, subunit 3, 130 kDa | 4.19 | CAB56791.1 | α1PV | |
| 4 | NCL | nucleolin | 11.97 | NP_005372.2 | α1PV |
| NCoR1 | Nuclear receptor corepressor | 3.20 | BAA75814.1 | α1PV | |
| SND1 | Staphylococcal nuclease domain containing 1 | 4.07 | NP_055205.2 | α1PV | |
| KIF11 | Kinesin family member 11 | 2.37 | NP_004514.2 | α1PV | |
| CCAR1 | Cell cycle and apoptosis-regulatory protein 1 | 2.26 | NP_060707.2 | α1PV | |
| TRIM28 | Tripartite motif-containing 28 protein | 3.83 | NP_005753.1 | α1PV | |
| 5 | VCP | Valosin-containing protein | 7.69 | NP_009057.1 | α1PV |
| SFPQ | Splicing factor proline/glutamine-rich | 7.21 | NP_005057.1 | α1PV | |
| 6 | XRCC5 | x-Ray repair complementing defective repair in Chinese hamster cells 5 | 20.31 | EAW70563.1 | α1PV |
| NCoR1 | Nuclear receptor co-repressor 1 | 1.57 | BAA75814.1 | α1PV | |
| DNAJC10 | DnaJ (Hsp40) homolog, subfamily C, member 10 | 3.40 | NP_061854.1 | α1PV | |
| PLOD1 | Procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 | 3.16 | AAH16657.1 | α1PV | |
| ILF3 | M-phase phosphoprotein 4 | 6.06 | CAA66918.1 | α1PV | |
| 7 | HSPA1B | Heat shock 70-kDa protein 1B | 20.59 | NP_005337.2 | α1PV |
| XRCC6 | x-Ray repair complementing defective repair in Chinese hamster cells 6 | 24.79 | CAG47015.1 | α1PV | |
| PRMT5 | Protein arginine methyltransferase 5 | 4.71 | AAB66581.1 | α1PV | |
| RXRB | Retinoid X receptor, β | 4.69 | NP_068811.1 | α1PV | |
| 8 | STK38 | Serine/threonine kinase 38 | 13.33 | NP_009202.1 | α1PV |
| TBLXR1 | Transducin (β)-like 1 X-linked receptor 1 | 10.31 | NP_078941.2 | α1PV | |
| RXRB | retinoid X receptor, β | 8.44 | NP_068811.1 | α1PV | |
| PRPF31 | pre-mRNA processing factor 31 homolog | 6.81 | NP_056444.2 | α1PV | |
| NONO | non-POU domain-containing, octamer-binding | 6.37 | NP_031389.3 | α1PV | |
| 9 | THRA1 | Thyroid hormone receptor α 1 | 15.85 | NP_955366.1 | α1PV |
| 10 | ANXA2 | Annexin A2 isoform 2 | 13.57 | NP_004030.1 | α1PV |
| NPM1 | Nucleophosmin 1 isoform 1 | 18.71 | NP_002511.1 | α1PV | |
| HNRNPA2B1 | Heterogeneous nuclear ribonucleoprotein A2/B1 isoform A2 | 9.09 | NP_002128.1 | α1PV | |
| 11 | ADNP | Activity-dependent neuroprotector | 4.45 | NP_056154.1 | α1PV |
| SET | SET translocation (myeloid leukemia-associated) isoform 1 | 14.48 | NP_001116293.1 | α1PV | |
| 14 | KIF11 | Kinesin family member 11 | 13.92 | NP_004514.2 | βPV |
| 15 | KIF11 | Kinesin family member 11 | 8.24 | NP_004514.2 | βPV |
| CCAR1 | Cell-cycle and apoptosis regulatory protein 1 | 2.35 | NP_060707.2 | βPV | |
| 17 | EIF4B | Eukaryotic translation initiation factor 4B (eIF-4B) | 18.66 | P23588 | βPV |
| XRCC5 | ATP-dependent DNA helicase II | 4.78 | NP_066964.1 | βPV | |
| 18 | HSPA1B | Heat shock 70 kDa protein 1B | 25.43 | NP_005337.2 | βPV |
| PRMT5 | Protein arginine methyltransferase 5 isoform b | 11.77 | NP_001034708.1 | βPV | |
| RBM10 | RNA binding motif protein 10 isoform 2 | 3.05 | NP_690595.1 | βPV | |
| XRCC6 | x-Ray repair complementing defective repair in Chinese hamster cells 6 | 6.34 | EAW60448.1 | βPV | |
| 19 | THRB | Thyroid hormone receptor, β | 17.79 | NP_000452.2 | βPV |
| EIF4B | Eukaryotic translation initiation factor 4B41 | 6.71 | AAH73154.1 | βPV | |
| 21 | ANXA2 | Annexin A2 | 18.29 | AAH23990.1 | βPV |
| NPM1 | Nucleophosmin 1 isoform 3 | 7.72 | NP_001032827.1 | βPV | |
| Ca | RIF1 | RAP1-interacting factor | 6.07 | NP_060621.3 | α1PV |
| CHD4 | Chromodomain-helicase-DNA-binding protein 4 | 2.04 | Q14839.1 | α1PV | |
| SPTBN1 | Spectrin, β, nonerythrocytic 1, isoform | 1.76 | EAX00138.1 | α1PV | |
| Da | RIF1 | RAP1-interacting factor 1 | 5.87 | NP_060621.3 | α1PV |
| SPTAN1 | Spectrin, α, nonerythrocytic 1 (α-fodrin) isoform 2 | 1.58 | NP_003118.2 | α1PV | |
| Ga | PUF60 | poly-U-binding splicing factor 60-kDa isoform a | 9.66 | NP_510965.1 | α1PV |
| HNRNPK | Heterogeneous nuclear ribonucleoprotein K isoform a | 12.28 | NP_112553.1 | α1PV | |
| CDC23 | Anaphase-promoting complex subunit 8 | 4.06 | AAF05755.1 | α1PV | |
| SYNCRIP | Synaptotagmin binding, cytoplasmic RNA interacting protein | 5.52 | AAD38198.1 | α1PV | |
| LMNA | Lamin A/C | 5.38 | AAH33088.1 | α1PV | |
| Ha | HNRNPH1 | Heterogeneous nuclear ribonucleoprotein H1 | 7.35 | CAG33059.1 | α1PV |
| Ia | EEF1G | Eukaryotic translation elongation factor 1 γ | 7.55 | AAH13918.1 | α1PV |
| TUFM | Elongation factor Tu, mitochondrial precursor (EF-Tu) (P43) | 10.40 | P49411 | α1PV | |
| THRA1 | Thyroid hormone receptor α 1 | 7.80 | NP_955366.1 | α1PV | |
| La | NPM1 | Nucleophosmin 1 isoform 3 | 10.81 | NP_001032827.1 | α1PV |
| SPIN1 | Spindlin 1 | 16.88 | AAG38112.1 | α1PV | |
| ANP32B | Acidic (leucine-rich) nuclear phosphoprotein 32 family, member B | 9.56 | NP_006392.1 | α1PV | |
| Cb | RIF1 | RAP1-interacting factor 1 | 4.25 | NP_060621.3 | βPV |
| Ib | ACTB | Actin, β | 18.21 | AAH08633.1 | βPV |
| Lb | SPIN2B | Spindlin-like protein 2 | 17.44 | NP_001006682.1 | βPV |
| NPM1 | Nucleophosmin 1 isoform 3 | 16.22 | NP_001032827.1 | βPV | |
| KCTD5 | Potassium channel tetramerisation domain containing 5 | 15.81 | NP_061865.1 | βPV |