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. 2011 Apr 28;25(6):1018–1026. doi: 10.1210/me.2010-0513

Fig. 2.

Fig. 2.

Localization of Lef1, AR, p63, and Ki67 in the embryonic murine prostate. Transverse sections of the embryonic UGS at E16.5 (A–E) and E18.5 (F–J) stained with eosin and hematoxylin (HE) (A and F) and labeled for Lef1 (B and G), Ki67 (C and H), AR (D and I), p63 (E and J), and the nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI, C and H). In the emerging prostate buds and distal tips, Lef1 is up-regulated (B and G, arrows), and AR is down-regulated (D and I, arrows). Lef1 expression is also present in the dorsal UGS mesenchyme at E16.5 (arrowhead) and in all pereipithelial mesenchyme at E18.5 (G). Scale bars, 100 μm. K, Schematic representation of the embryonic UGS. bl, Bladder; ur, urethra; SV, seminal vesicles; AP, anterior prostate buds; DLP, dorso-lateral prostate buds; VP, ventral prostate buds. The dashed line indicates the plane of transverse sections in A–J. L, Graphic presentation of the relative percentage ratios of proliferating cells positive for Lef1 (Lef1+;Ki67+/Lef1+, black bar) and AR (AR+;Ki67+/AR+, white bar) in the embryonic prostate epithelium. P < 0.05. See Supplemental Fig. 1 for E17.5 UGS sections labeled for Lef1 and Ki67.