Fig. 4.

SXR regulates lymphocyte proliferation and apoptosis through NF-κB signaling. A–D, Loss of SXR increases splenic expression of pro-proliferation and antiapoptotic genes and decreases splenic expression of proapoptotic genes (D) as measured by qRT-PCR; E and F, loss of SXR increases splenic expression of proinflammatory SXR target genes as measured by qRT-PCR; G, qRT-PCR of WT spleen tissue after 3 d dimethylsulfoxide (DMSO) or PCN treatment (mean is indicated as fold of vehicle); H, cell proliferation as measured by DNA content. The 24- and 48-h treatments are shown, with dimethylsulfoxide, SXR ligand rifampicin (RIF), and 1 and 2 μm CAPE treatment for each. Values are expressed as fold of 24-h dimethylsulfoxide.