Immunohistochemistry for SF-1, Ubc9, and PIAS1 in human adrenal glands. Sections show hematoxylin and eosin staining (A) and immunohistochemical stainings of SF-1 (B), Ubc9 (C), and PIAS1 (D). Zona glomerulosa (ZG), zona fasciculate (ZF), and zona reticularis (ZR) are shown in A. Original magnification, ×20.