Immunohistochemistry for SF-1, Ubc9, and PIAS1 in an aldosterone-producing adenoma (A–D) and a cortisol-producing adenoma (E–H). Sections show hematoxylin and eosin (H&E) staining (A and E), SF-1 expression (B and F), Ubc9 expression (C and G), and PIAS1 expression (D and H). Original magnification, ×20.