Fig. 5.

Characterization of chimeric prostate tissue from normal human prostate progenitor cells. A, Increasing numbers (100–10,000) of prostate progenitor cells mixed with a constant amount of UGM produced grafts of increasing size. B, H&E staining of a 1-month graft shows normal glandular structure with prostatic histology. C, Immunofluorescent labeling with antibodies against p63 (green-aqua) and CK8/18 (red) with 4′,6-diamidino-2-phenylindole (DAPI)-stained nuclei confirms differentiation of cells into prostate basal and luminal epithelial cells. D, Nuclear immunostaining of AR (green) further confirms prostatic cytodifferentiation of the epithelium. E, The human origin of the epithelium in chimeric grafts was demonstrated by immunolabeling with human-specific antinuclear antigen (red stain; pink when merged with blue nuclear DAPI) and DAPI-labeled nuclei (blue) at 1 month. Note that stromal cells are negative for human nuclear antigen. F, Human origin and functional differentiation of the epithelium were further confirmed by immunostaining for PSA (red) in the luminal cells with DAPI-labeled nuclei. Scale bar, 50 μm.